LOCUS       EY190862                 422 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07b07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190862
VERSION     EY190862.1  GI:159493230
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 908 was extended to value 909 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 615
            PolyA tail found inside read...SR being truncated to 573
            Potential full insert read...vec-to-vec length: 561
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa caacttgaat tttgcgactg taacagagat caaaattgga tttcaaagtt
       61 tccattacca acagctgacc caggcccaga aggacctgaa catcgtcgcc ttcatccgac
      121 gggcgatgga aggaaggtcc aaaggggaga aacgcaatgt gatttacgag aatcgctttt
      181 atttccaggt gaaaggaacc agtgtagtca atccaccggg cggcccaatc gagaccgaaa
      241 ttctctacac tgaaagcggg cagtatgcga gtctttgaga agatctataa ttatagaaga
      301 ggaaataaag agttttgtcc attgatgttc cgaagcaagc taagcgaaat aagagaagat
      361 gaccgttgat aaaacgttta tttcccgttt gttgatcaga ataaagtcgg tcgaagtgta
      421 ga
//