LOCUS       EY190861                 389 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07b06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to pir|T25713 hypothetical protein F19F10.9 -
            Caenorhabditis elegans, mRNA sequence.
ACCESSION   EY190861
VERSION     EY190861.1  GI:159493229
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 901 was extended to value 902 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 582
            PolyA tail found inside read...SR being truncated to 541
            Potential full insert read...vec-to-vec length: 508
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggccg gagaaagcgg agtatgcgcc caaaatcgag atcaactacg cggatcagaa
       61 agggcgcatc ctcgactcaa aagaggcatt ccgagagctc tcgtggaaat tccatggcaa
      121 agggccaggg aagaaacagg cggagaagca cgcagcaaag gtggtcaaga gggagaagct
      181 gaaaaagatg aattcaagcg acactccact ggggacgctg aacaaacagc tgaagaagca
      241 agaacagttg cagactccct acctcgtcct gagcggacgc ggagaagggc aacctctcca
      301 aaaagaatga gtgggaggag actcctaggg gatgttttga aatgtttcca tttttctctg
      361 accatctccc aagtaaaata tgtgataga
//