LOCUS       EY190859                 313 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07b04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190859
VERSION     EY190859.1  GI:159493227
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 873 was extended to value 874 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 506
            PolyA tail found inside read...SR being truncated to 464
            Potential full insert read...vec-to-vec length: 453
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggag ggacccgatt acgatttcgg gcctgttcaa tttcgacaag aacagagtga
       61 tgccggaaga atggcacgag tactggtaca aagaggcata ctttgattca attgcctact
      121 cctctcgctg ccttgaccca ctcggaaaat cgtacgaagc cataaaggat ccacaccgaa
      181 aaaggaaagt cacgcatcat taggaagaaa cagcaatcag acaaataaaa agacttcaaa
      241 ttgttaggga caattttgta cattgatcca tgtcgctgat cacattcatt cgggaataaa
      301 gattgatagt tga
//