LOCUS       EY190855                 491 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa07a09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_504501.2| Pellino, Drosophila Pelle associated
            protein homolog (51.8 kD) [Caenorhabditis elegans] gb|AAB37081.2|
            Hypothetical protein F25B4.2 [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY190855
VERSION     EY190855.1  GI:159493223
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 883 was extended to value 884 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 685
            PolyA tail found inside read...SR being truncated to 642
            Potential full insert read...vec-to-vec length: 563
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg aaaaaatgac acatttcgct gcccaatctg catggtcgat tcggaccgtg
       61 ttgcccaact gacgatggga atggaaccgg ctttccacct cgactccagc tcactcgact
      121 acgccttcaa cccgtgtggc cacgtttcct cgttgcggac cgttcgatat tggtcgagaa
      181 tcccccttcc acatggtacc aacagcttct acccggtgtg tccgttctgc acttctctgc
      241 tctccgtcga aaggccattc gtgcgactca ttttccagga ccattgccac gagtaacaaa
      301 caaaggtaat ttccagcgat caaagaacct cgtaaagttg tggaataata ataatctgag
      361 cgacccattc ttgtaatgtg tgacctgcat tcttctttcc tatacatacg attcagacaa
      421 ttttctctgt accaaagtga cactttcaaa aacaatatga attcgccagt aaagccgtct
      481 tttattcaat a
//