LOCUS EY190835 541 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa05g09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to ref|NP_491475.1| NHL repeat [Caenorhabditis elegans] pir|T25723 hypothetical protein F21F3.1 - Caenorhabditis elegans, mRNA sequence. ACCESSION EY190835 VERSION EY190835.1 GI:159493203 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 541) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 937 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..541 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggcaa caaacacttc tgcaagccaa ccgatgtggc tgtggccagc accggcgagt 61 tcttcatcgc agacggctac tgcaacagtc ggatcatgaa gttcgatcgg gacggcaacc 121 tgctcagcca gttcggccac cctaactcgg aagagccgcc gaagaacggc ggattttttg 181 tgccgcactc gattgcactg attgagaacc tgaacctgtt gtgtgtggcc gaccgcgaga 241 acgaacgcat ccaatgcttc tctgccggac tggaaggtag cgctgcccag cgcaaactgc 301 atcggcgtgc gtacgtccca acgggcacat tcgtcaccaa ggcggagaac atcggacgcg 361 tgttcgccat tcgcgaaaaa gaacacttcc tcaccggtgt cactagcccg gacagaaatg 421 gccaactgga gccacaagtg ttcgtcatgg acatgaacac cggacgcgcg aacactttcg 481 ccaagggtct ggagaatgcg cacgcgatcg cgttgagcga caacggggac atctttgtgt 541 c //