LOCUS       EY190827                 518 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05f06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190827
VERSION     EY190827.1  GI:159493195
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 911 was extended to value 912 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 710
            PolyA tail found inside read...SR being truncated to 669
            Potential full insert read...vec-to-vec length: 636
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat atgtactttg aggacaaagt gcgcggtcga aaaatctcga gaagtggcct
       61 tacatggttt ctcttcaaag tgcgaatgta ccttagctac cggtcctcgt acaatcattt
      121 cgagactgtt ggcacagcaa ttcgctttga cgaaaatatt gtgtcgctca tttggcgttt
      181 ggactcgaac aaatcgactt tgaggaatta ttttcaatca cccctgttga tcaagaaagg
      241 acttaatgcg ttaaatgaaa ccatttctga aattcaatcg gaaaagccaa tggatgggaa
      301 ggaaaggatc atgatggaga agaaagtcca agaaatgatg agagttcccc gcaccaaggg
      361 tttggagatt gaggagtgtg tggacatcca cgtgaacgaa ggcggccgag tggaccgaat
      421 tgtgaaccgg aaagcggata aaagcgatta tgaactggcg gaaaagagag cggtggtgtt
      481 ggcggcagag ctggaggaag agttgaggaa acagcgca
//