LOCUS       EY190825                 362 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05f04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190825
VERSION     EY190825.1  GI:159493193
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 881 was extended to value 882 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 555
            PolyA tail found inside read...SR being truncated to 513
            Potential full insert read...vec-to-vec length: 480
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac cgaaaggacg agaggagtgg ttctcgctcc gggagtcgct ccgtgtccag
       61 gagtggctcg cgcggccggg acagggatgc cagccggggc tcacgttcga gcagcaggtc
      121 gcgctcagcg agcaggtccc agtccggcgg gagccggtcc agctcgagga gctcgtcccg
      181 ctccagcagc ggctctcggt ccagcaacag atcggcgtcc gagggcagat cgcagtccaa
      241 tcacggcaaa tcgaaggcgt ccaccaggga caaaaagcat tcgtccaagg acaaaaagtc
      301 gagttcttcc aaggacaaaa aatcgcacac ctccagagag aagaaggcgc atgactcagg
      361 ca
//