LOCUS       EY190824                 460 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05f03.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190824
VERSION     EY190824.1  GI:159493192
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 914 was extended to value 915 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 655
            PolyA tail found inside read...SR being truncated to 611
            Potential full insert read...vec-to-vec length: 599
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtcc gaataactgg caaatggagt tgaactaaaa tgcgcagccg cacatgcagt
       61 atttgatcaa aaagaggcta agtcgagctg atcccaattg cgatagcctc tagcggggtc
      121 gacggtctcg actctcgaca gcgcggcagc cgacggaatg gacaaaaaca gcaatgatca
      181 acctgatcat ggggattcgc gaccagtgag cgaacaacag caacagcttt tcgcgccttc
      241 ggcacccttc gatgagtgag caaattccaa catataattg ccgaaaacag attggatgtg
      301 ccgtgccatt cccaacggcc cagggctcca gaagtattcg ccgattccaa cttccaagcg
      361 ccgacaaata tttttgccaa acgccgttat taggccgaag gctggatatt actcttttgg
      421 aaacagcttt gtttgatatc gatagaaaaa atgttgtcca
//