LOCUS       EY190823                 349 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05f02.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190823
VERSION     EY190823.1  GI:159493191
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 922 was extended to value 922 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 156
            original value before phred recall for SR was 550
            PolyA tail found inside read...SR being truncated to 507
            Potential full insert read...vec-to-vec length: 467
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga ttggtacatg cttaaaaaaa attccaggaa atagcactta aatatttatg
       61 aggccgtacc gtaaaccgac actggtggat tggtagagta tactaaggtg ttgaaagaat
      121 gatgttgaag gaactcggca aattatacct gtaacttcgg gagaagggta acctattttt
      181 aggtaactat taataggtgg cacaaagtag ggagtggcga ctgtttacta gaaacacagg
      241 actctgcaaa cacgtaagtg gaagtatagg gtctgacgcc tgcccggtgc tggaaggtta
      301 ataggagggg tgaaggctct gaatcgaagc cccagtaaac ggcggccga
//