LOCUS       EY190822                 584 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05f01.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_501914.1| Isoleucyl tRNA Synthetase IRS-1 (130.0
            kD) (irs-1) [Caenorhabditis elegans] sp|Q21926|SYI_CAEEL
            Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
            (IleRS) pir|T24176 hypothetical protein R11A8.6 - Caenorhabditis
            eleg, mRNA sequence.
ACCESSION   EY190822
VERSION     EY190822.1  GI:159493190
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 898
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggtgc caagcaacat ggctctttgt gtgcatcctg agtacgatta catcgtcgtt
       61 gcggacaaaa aatcgggaaa agaattggtc atcttgaagg aacggctagg cgaattatac
      121 aaaactgcgg attcttatac ggtggtcgag gaactcaaag gaaatgcact caaagatatg
      181 gagtacactc cgcccctgcc ctactttctg catctcaagg cggagggcag aaaggtcttc
      241 cgagtgttga cgaacactta cgtgaaaacg gaccaaggca cgggaattgt tcaccaagcg
      301 ccctatttcg gtgaggacga cttcaagaca tcgctggaac atggcgtcat cagcaaggac
      361 tcgaagatga tctgcccagt ggatgggaac ggaaggttca cggaggaagt ggccgacttc
      421 aaagggctct atgtgaagga cgcggacaag ctgatcatca agctgctgaa ggagaggggg
      481 ctgctgataa agcaggagca gtacaagcac agctacccgt actgctaccg ctcacagacg
      541 ccgctcatct acaaagcggt tccgtcgtgg ttcattcgcg tgga
//