LOCUS       EY190821                 443 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05e12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190821
VERSION     EY190821.1  GI:159493189
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 868 was extended to value 869 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 664
            PolyA tail found inside read...SR being truncated to 595
            Potential full insert read...vec-to-vec length: 561
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcaa gtggagatgg cctctgttga gcagccgaag aagatcgagt acatcaaccc
       61 gttgttggct gagaagagca acggggaagt gcaggactct gacgcggcaa atgggaagac
      121 gccaacgcgg cggcacaacg gtgcaacgcc aaacaacgat tgggcgggga ttagactgcc
      181 gccgccggta ccgccaaagc cgaaaataaa gatcaacatc acggccagcg attgttgcat
      241 tcacacgaga gaaatcaaat aatgaagtac aagacatggt aaatatttgt gtacgcatca
      301 aacaacatca gttgtactaa attgtatagt aactcttaat tcactcagac ttttatctat
      361 tttccaccca ttttcagcta taacacttcc acattacata ttttattgat tattgcctcc
      421 ggataaatga tgtctgtctt cca
//