LOCUS       EY190820                 430 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05e11.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190820
VERSION     EY190820.1  GI:159493188
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 896 was extended to value 897 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 624
            PolyA tail found inside read...SR being truncated to 582
            Potential full insert read...vec-to-vec length: 568
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggac gtggtgcccc cgtgcccgaa ttcgtccgca ccgtctgtgt acgcggaacc
       61 cgcaaaaatc gcacccattc cggccaagcg gaacggctca gcggtgaacc acttcagcaa
      121 tgcggcgttc gacaaccaac tctacaacat ggatggggtg gcggacggca cgccggcgga
      181 ggagaaggaa caccggaatg ccataaggcg ccagatcgag gcgatggaga cggcggacaa
      241 ggaacagcac aaggaagagg gcaccaacgg catcagcctg ccttcgtcca acgagtccag
      301 cccctacaag gacatatatc tgcacggctg agggcagaga agggaacgag cactaacaat
      361 aacaacaaca catgataata taataattat ttgtcaagtg ataacctggg taccgccaaa
      421 tatatagata
//