LOCUS       EY190819                 414 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05e10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190819
VERSION     EY190819.1  GI:159493187
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 921 was extended to value 922 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 607
            PolyA tail found inside read...SR being truncated to 564
            Potential full insert read...vec-to-vec length: 486
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggt gacttactcc aagcgggcca ccatcaagat gacgcgtgag gactgcgcta
       61 ggctggagga ggcgcgcaag aatgcgatca tccgggagct gcaggcgatg gagcagcagt
      121 cgaagaaacg ggggattggc ctcctcctca agaacgactc tgatgagacg gctaatggca
      181 ccggtgctgg aggggcagag caccactgat gaaagaaaca gcagcacctg catctaaatt
      241 ggcctggaaa ttgtgagagt gctcgcattt ttcttttgga ttctggatat gatcttttct
      301 ctcccaatcg tctgtttatc ttcaagtttc gaatgttcac gagtctccag ttgaagtcgt
      361 gtttcagctt ttgactatgt catttcaatt ttcgaggata aaaaaggatg ctga
//