LOCUS       EY190818                 301 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05e09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190818
VERSION     EY190818.1  GI:159493186
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 877 was extended to value 880 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 494
            PolyA tail found inside read...SR being truncated to 451
            Potential full insert read...vec-to-vec length: 373
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggt tataaataat taatgaagta taaactatta atgttatatt ttttagataa
       61 attaattttt ctagtaattt atttttagat aaattaatgt tccagaaata tcggctagac
      121 attattattt ttaactaaaa actttttaaa ttttatttta atttatataa atttatatta
      181 atataggtga aattttaatt ataattatgt taataatttt ataaaattta aaattttaat
      241 ttttaactta ggttagacac taattaatga aatttaataa ttttctttag taaatttttg
      301 a
//