LOCUS       EY190814                 482 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05e04.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to sp|P92504|CYC1_ASCSU Cytochrome c, type-1, mRNA
            sequence.
ACCESSION   EY190814
VERSION     EY190814.1  GI:159493327
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 846 was extended to value 847 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 673
            PolyA tail found inside read...SR being truncated to 632
            Potential full insert read...vec-to-vec length: 554
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg aaagaaagtc ttcaaggccc ggtgtctcca atgccacgtg atcgactcca
       61 tggccacaaa gacaggacca accctgaatg ggatttgggg aaggaaaagt ggacaggtac
      121 ccggattcga ctattctgcc gccaacaaaa ataagggtgt tgtttggaat cgcgaaacgc
      181 tcttcgaata cctgctcaat ccaaagaaat atatcccggg gacgaagatg gtttttgcag
      241 ggctgaagaa ggtcgacgaa agggcggatc tcatcaagta catcgaggtt gaaagtcaga
      301 aaccgccagc atgatgaatt gacgcatgag aggttcatcg cccgtccacg acaaacagga
      361 tagtttatag tcagtcacgt gcgagacact tggttaaaat tgtttttcac ctagacattt
      421 tcacactctg aggccctcgt ttctaagaaa ttgttagttc gaaaaaataa aattcgttga
      481 ca
//