LOCUS EY190808 127 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa05d10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190808 VERSION EY190808.1 GI:159493321 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 127) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 847 was extended to value 848 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 322 PolyA tail found inside read...SR being truncated to 277 Potential full insert read...vec-to-vec length: 199 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..127 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggaac tcgaatgctg cgtaacttgg aaaagattaa atggattcat tcgcatgaat 61 tgatgttttt gcaggaatcc tttttatttt ctgttatttg aaattcgata aaccaatttg 121 ttgcgga //