LOCUS EY190804 460 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa05d06.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to gb|AAA26471.1| csgA, mRNA sequence. ACCESSION EY190804 VERSION EY190804.1 GI:159493317 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 460) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 927 was extended to value 928 (,) This trace has been recalled with phred original value before phred recall for SL was 150 original value before phred recall for SR was 654 PolyA tail found inside read...SR being truncated to 612 Potential full insert read...vec-to-vec length: 597 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..460 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggat gtgaacctgg tgggcactgt gatgctcacg gcagcccttc tgccgttgct 61 aaagaaggcg gcgagtgcgg gggagcacgc gcgagtggtc aacattggct ccgcactggg 121 ctgcatcgca aatacgccga agccagcgat ggcgccgcat cgtaatgtgg cctatgggat 181 gagcaagcaa tgcatcactt caacaagcaa cttgcactcg gtgagccaga catcactagc 241 gtggccattc atcccgggtg ggtgcgaaca gaaatggggg gtccacaggc tccaatcgcg 301 gtggaacagg cggcacaggc catattgagg cgagtttgcg cgctgaggaa agacgactct 361 ggcaaactat tggaccacga aaaggaattg gccccgtgaa aatttccact agcagattaa 421 tttattcaca actcttatta taaaattcgg atttttggta //