LOCUS EY190800 337 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa05c12.g1 R.similis_EST_RSAA Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190800 VERSION EY190800.1 GI:159493313 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 337) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 884 was extended to value 884 (,) This trace has been recalled with phred original value before phred recall for SL was 151 original value before phred recall for SR was 532 PolyA tail found inside read...SR being truncated to 490 Potential full insert read...vec-to-vec length: 468 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..337 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac atacagccca gccagtcgcg actctcattg ctcgctttaa atgcgaccaa 61 gccctaccac agtggcggtc ttaacacttt ctcttccacc agccgagccg acgatataga 121 tagatgtcgt gagtgaggga tgaaaaggag gcatggaatc agcacggagt gggaacggca 181 agggacgaca tttatagccg atgtataaaa ggaatagggg aaatatgtat ggagggatag 241 ggattttagg ataaagtatg ttttactgga ccactcgaat tttcgcataa aaacaatttt 301 atgttacata cctatgtatt tttaggcgca ataaaga //