LOCUS       EY190798                 469 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05c07.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190798
VERSION     EY190798.1  GI:159493311
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 909 was extended to value 909 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 148
            original value before phred recall for SR was 662
            PolyA tail found inside read...SR being truncated to 619
            Potential full insert read...vec-to-vec length: 608
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcat gttattggcc aatactgggt ttgacctcta cttggtccct gagaaactgc
       61 aaccgaacag ccagactctc gtgaatgccg gccttgcgcc aaaatgcagc atattggtca
      121 agttcacaga cgtgacattg aatgcggttg agggattgct aaggatggaa ttgattctct
      181 gtggcaccga ggaaacagca aaactaactg ccgacaagtg gctcggggtc aacacgctat
      241 accgcccgta cctgtggaca ttggcacaag agttggtgcc aaatgaccca atgaatggga
      301 aaatatccgc taacgcttcg gccgccggac gtgatgttgt tgatgaggag ggatcaaaga
      361 gagagagcag acatctgccc gcaaaccttc cgaagtggtt caaatgaaac aatcaataaa
      421 ccaatgtact attttattat aagttattaa aatcatttgg agcgtaata
//