LOCUS EY190791 201 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAA-aaa05b10.g1 R.similis_EST_RSAA Radopholus similis cDNA 5' similar to sp|P23381|SYW_HUMAN Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TRPRS) (IFP53) (hWRS) pir|A41706 tryptophan-tRNA ligase (EC 6.1.1.2) [similarity] - human emb|CAA42545.1| 471 aa polypeptide (gamma2) [Homo sapiens] gb|AAA67324.1| transf, mRNA sequence. ACCESSION EY190791 VERSION EY190791.1 GI:159493304 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 201) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 911 was extended to value 911 (,) This trace has been recalled with phred original value before phred recall for SL was 149 original value before phred recall for SR was 397 PolyA tail found inside read...SR being truncated to 352 Potential full insert read...vec-to-vec length: 338 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..201 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022111 R.similis_EST_RSAA" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggag gaaaatagcg atcgagctgg tgcaagggat cgtggccgat ttgcagcaac 61 gccgcaaaga agtgaccgat gaggtgctgc gggagttcat gaccccgcgg aaactggctt 121 tcgatttttg acgacccaca tttttgtcca agaaccaatt ttgtgtgaaa tgtgcatgca 181 tgaaccggat cacgaccagt a //