LOCUS       EY190789                 659 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05b08.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to ref|NP_492113.1| Chitin synthase CHI-1 (chi-1Co)
            [Caenorhabditis elegans] pir|T25284 hypothetical protein T25G3.2 -
            Caenorhabditis elegans emb|CAA96688.1| C. elegans CHI-1 protein
            (corresponding sequence T25G3.2) [Caenorhabditis elegans], mRNA
            sequence.
ACCESSION   EY190789
VERSION     EY190789.1  GI:159493302
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 928 was extended to value 928 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 933
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggct tctcgatgga gtccaatgtg cagctgatgt acgccaaaat agcgtcggtc
       61 atctacgcat tcatcatgct ggccgtgctg atcgctacca ccaatcaaat cgtgttggag
      121 acggtgttct cgcccacgtc gatgttcgtg ctgggaatgg tgtgcatctt ctccttcgcc
      181 tcctgcatcc accccaaaga gttctccaac atcgtcttcg gcacggtctt ctttctgatg
      241 atcccctcca cgtacgtgtt cctctccctc tactccctga tcaatctgaa cgtgatcaac
      301 tggggcacac gagaggcggt tgccaaagct acgggacaag tctcagagag ccttgcgggg
      361 cgcatgttgc gccgagtgat caacaccagc gacgacagtt cggccttggc gcgcttgctc
      421 ggtcgatttc gcaccagcga agagtccaac gaaaaaatcg tcacattgga gaagaagctc
      481 gagcgggtgg aaggaatact gtcacaaatg aatgatgaaa gtgctctggc ggatgggatg
      541 gagggccaag acgggcagat gatcaacatg ctggacctgg agagtgagga ccaaagaagt
      601 ccgccaagaa ggagatatga ggcggtagca attcgacaac ttggtgcggg tgacggcac
//