LOCUS       EY190780                 464 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05a09.g1 R.similis_EST_RSAA Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190780
VERSION     EY190780.1  GI:159493293
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 919 was extended to value 919 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 651
            PolyA tail found inside read...SR being truncated to 616
            Potential full insert read...vec-to-vec length: 602
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggt tgactactac gcactcttta atcatggcgc aaccagagtc cacatcaaga
       61 cagggggcac aagtgtacaa gatcggcgag atccacgccg actgcacttt ttcgacccat
      121 ttcaatcaat gaattcgacg cgcgctcgtt ttgctggtga cgctttaaca gtcgctttcc
      181 aatttttacg caatcggata ttcactggtc gccatactct cacttttcca cgattggacg
      241 ggctcacgga cgattttaca aaagaagtta caagtcaggg ccgccaatgg acgaagtcgt
      301 tggatccatc taatgatatg ggacggcaag tgcaagtaca atagaaggaa aagaaatcga
      361 agaggatggt attgatatcc tgatgtctga tacggcttcc tatgttgaaa aaagatttca
      421 tattcatgaa gtgctcgcta tcggaaatac gtacattctt acta
//