LOCUS       EY190777                 319 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAA-aaa05a05.g1 R.similis_EST_RSAA Radopholus similis cDNA 5'
            similar to sp|P19234|NUHM_RAT NADH-ubiquinone oxidoreductase 24 kDa
            subunit, mitochondrial precursor pir|A31868 NADH2 dehydrogenase
            (ubiquinone) (EC 1.6.5.3) 24K chain precursor - rat gb|AAA41669.1|
            24-kDa mitochondrial NADH dehydrogenase precursor (EC 1.6.9, mRNA
            sequence.
ACCESSION   EY190777
VERSION     EY190777.1  GI:159493290
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 832 was extended to value 832 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 513
            PolyA tail found inside read...SR being truncated to 470
            Potential full insert read...vec-to-vec length: 437
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022111 R.similis_EST_RSAA"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggat gcacgaggtg gagtgtctcg gtgcctgtgt caatgcccca atggtccaga
       61 tcaacgatga ctactatgag gatctgaccc cgagggacat tgaggagatc atcgacgacc
      121 tgaaggctgg gaaacgacct ctgccagggc cgaggagtgg ccgattggct gcagagccac
      181 tcaccaagaa aacgtccctc acagaaccac caccggggcc tggacatgga ttgcagccag
      241 gactgtgaac aaatagaaga tttcaccttc tttaaatctt caatgcaatt gtagaatttt
      301 ggataaattt taagcccga
//