LOCUS EY190771 217 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa04b10.g2 R.similis_EST_RSAB Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190771 VERSION EY190771.1 GI:159493284 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 217) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 517 was extended to value 517 (,) This trace has been recalled with phred original value before phred recall for SL was 152 original value before phred recall for SR was 522 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..217 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggggg ttcagccatc atgattagtc gttctaaccc cattcgaagg cattaagtat 61 tactggtcgc cttctggtgt tttacaaggt catatacatg ctggtcggtg atgagagcgc 121 aaatgccgaa tgttttgagg tgcgtagata cgggggaggg aaacgggcgg aaaaacacct 181 tccgcttgtt tgattgggtt aacaccattt ccatatc //