LOCUS EY190751 173 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa02d05.g2 R.similis_EST_RSAB Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190751 VERSION EY190751.1 GI:159493264 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 173) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 966 was extended to value 967 (,) This trace has been recalled with phred original value before phred recall for SL was 156 original value before phred recall for SR was 417 PolyA tail found inside read...SR being truncated to 324 Potential full insert read...vec-to-vec length: 308 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..173 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccgggtct acaatgtgca catgacaaaa gcgagggata tggataaggc gcacattcag 61 gcctaaaagc ggatagaagc cattttgacg ctttataact gatagtgaat actaaaaaca 121 tgaatgtcaa tatttctcca aattttatgc agcgataaaa gttattctct tca //