LOCUS EY190739 286 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa02e09.g2 R.similis_EST_RSAB Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190739 VERSION EY190739.1 GI:159493414 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 286) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 902 was extended to value 903 (,) This trace has been recalled with phred original value before phred recall for SL was 155 original value before phred recall for SR was 485 PolyA tail found inside read...SR being truncated to 441 Potential full insert read...vec-to-vec length: 424 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..286 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggtt ctaacaagtt gcatcctttt cccccgaaac atggctggct catgcatatc 61 ccctactcta ctactttttg tttgatgctt gactttttga taatattgac aggattgaaa 121 ttttgattaa ttgccaacat ttcccccaca gaaattgtga tgaaatgttc ttgttgacca 181 tttaaaggct tttatcatca tctttcatcc cgaccgattt ccatgtttct atcgtcatta 241 gtacatttga aatttgcttt tttttataaa tgaactaatt tgttga //