LOCUS       EY190738                 475 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa02f05.g2 R.similis_EST_RSAB Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190738
VERSION     EY190738.1  GI:159493413
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 915 was extended to value 915 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 669
            PolyA tail found inside read...SR being truncated to 625
            Potential full insert read...vec-to-vec length: 613
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa gagcaacaga agctgcacga ggaggcccac aaggagatgt tcgaggacga
       61 ggactacggc ttccccgcag tgccgcagca gcctgtggag gactcgcctc tgtcctcggc
      121 ttacgccgac tcggaggaga tctactccaa ccgcagctac ggaggggaga gcgagggcct
      181 ctcttctggc ttcggaagcg acgagaccga cccgatcccc gaggtgaact cgtcgtacaa
      241 gagccctgaa ctgctgtccg aggccgacga gtacaaaatt gccgaggacg ccaaaatggc
      301 cgccatcgac gacgacaaga tggacaactt cgtctgaagt ggagcacaga acggaaagtg
      361 gaaaagggca aacggggaga atggatgagt ttagaggatt gaagggcatt tgaaaaaaaa
      421 gatttcaaat aaaaggggtt gtggattcat ggcatattgt gaaaaaattt gagca
//