LOCUS       EY190733                 323 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa02g12.g2 R.similis_EST_RSAB Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190733
VERSION     EY190733.1  GI:159493408
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 523
            PolyA tail found inside read...SR being truncated to 478
            Potential full insert read...vec-to-vec length: 463
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga gggacctgtc agtgtgttcg tccgattcaa accggaggtg tatgtgcccg
       61 ttttccagga agaattgcgc caacaatttg tggacccact caagtttgcc cgcgaaaagg
      121 atgcggaagt gcgtgaaaat tttgccaatc cgatgcgact ttccggcgct aaaaagaaag
      181 ttggacttaa aacggaagtg ataaatggaa aatattggac ggaagagaga accgacccca
      241 aataaattgt ttaggaattt ctaaaatttt ccgcagaaat ctattaattt tgcttagaat
      301 acctagaaaa tgaagtgaat tga
//