LOCUS       EY190728                 331 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa02f01.g2 R.similis_EST_RSAB Radopholus similis cDNA 5'
            similar to ref|NP_503542.2| Lipase class 3 precursor family member
            (38.5 kD) [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190728
VERSION     EY190728.1  GI:159493403
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 526
            PolyA tail found inside read...SR being truncated to 482
            Potential full insert read...vec-to-vec length: 469
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcac ggggaggact tcaactgcag cgaccaactg gcctttgagc tgaccaagta
       61 ccagacctac atttgggacc accgccacta ctttgggcac aaggtgccca cctggggcaa
      121 aggcggttgt gtcgcggagg cgaacagcgg caacggcacc agcagcgccg agtccacgtc
      181 aagcgttgac tgaacaagaa aagaaaaaga ggagggagtg gaccagtgga cagtggatga
      241 atgggtgtag tgaacgccca gacagcgaca ccaaatccca aatgcttctt ggagtgtttt
      301 cggcacttta ttgatctggc cttccatcat a
//