LOCUS EY190720 330 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa02g01.g2 R.similis_EST_RSAB Radopholus similis cDNA 5', mRNA sequence. ACCESSION EY190720 VERSION EY190720.1 GI:159493395 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 330) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center original QR value of 906 was extended to value 911 (,) This trace has been recalled with phred original value before phred recall for SL was 148 original value before phred recall for SR was 523 PolyA tail found inside read...SR being truncated to 479 Potential full insert read...vec-to-vec length: 405 Seq primer: -21UPpOT. FEATURES Location/Qualifiers source 1..330 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 ggccggggac agtgtgctcc agaagtacac caacagcgag gaaatgatgc gcgccatcgc 61 aaaatgatag aagacgtaga ggggctgctg agtccggagg gaatgtacac cgacatctac 121 gcaaacactc cgccgatccg aattcgaggc atcacgcccg gggacagtgt gctccagaag 181 tacaccaaca gcgaggaaat gatgcgcgcc atcgcaaaat gatagaaaac gtagaggggc 241 tgctgcaatt cgggatttca ctcataaatt atttggacaa cgctgtttgg tcacggaaaa 301 agcagtacaa tataaattga gaaaatgtca //