LOCUS       EY190713                 664 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa02h12.g2 R.similis_EST_RSAB Radopholus similis cDNA 5'
            similar to sp|P27443|MAOM_ASCSU NAD-dependent malic enzyme,
            mitochondrial precursor (NAD-ME), mRNA sequence.
ACCESSION   EY190713
VERSION     EY190713.1  GI:159493388
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 871 was extended to value 872 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 875
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggc gcctcgaccg tgaggggagc gttcacccac ggcatcatcc gcaaaatggc
       61 cgagttcaac cagcgcccga tcatctttgc actctccaat ccgaccagca aagccgagtg
      121 cacggcggag gacgcctatc gactcacaaa tggaactgtg cttttcgcat cgggcagccc
      181 cttcgagaac gtggaactgc acggtcgcgt cttcaaaccg ggccagggca acaacgcgta
      241 catattcccg ggcgtggcgc tggccgtcat cctcttcaaa gtgtccacca tcgacaacaa
      301 gctgtttcta ctggcggcca gaacagtcgc agagtcagtg accgagaaga gtttgtccga
      361 aggccggatc tatccccggc tgaaggaaat ccgggaactg tccatcaaga ttgccatcaa
      421 actggcggaa gagtgctaca gggacgggac ggcaaagctc taccccgaac cggaagacaa
      481 ggaaatgttc atccgctccc aggtctactc agtcgaatac gatgacctga tcaacaagac
      541 ctacgactgg cccagccagg acatgcgcca tggctttccg atcccaccgg tgcgtcgtga
      601 cagcatggac gactaaataa tagctcaata aaatggatat ttatatcgca aagatgatcc
      661 tttc
//