LOCUS       EY190700                 324 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa02f06.g2 R.similis_EST_RSAB Radopholus similis cDNA 5'
            similar to gb|AAK72124.1| beta-actin [Chrysophrys auratus], mRNA
            sequence.
ACCESSION   EY190700
VERSION     EY190700.1  GI:159493375
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 948 was extended to value 948 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 152
            original value before phred recall for SR was 577
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggct ctctggcggc accaccatgt acccgggaat tgccgaccgc atgcagaagg
       61 agatcaccgc tctggccccg tcgacgatga agatcaagat catcgctcct cctgagcgca
      121 agtactccgt ctggatcgga ggctccatcc tggcctcgct gtccaccttc cagcagatgt
      181 ggatcagcaa gcaggagtac tacgagtcgg gcccgtccat cgtgcaccgc aagtgcttct
      241 aagcagggac agcggacagc gagacagaca cagaggaagg cagacagaca gacaacagaa
      301 aagtgcttct tttccaccct tcca
//