LOCUS       EY190689                 539 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa02g06.g2 R.similis_EST_RSAB Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190689
VERSION     EY190689.1  GI:159493364
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 651 (,)
            original QR value of 889 was extended to value 890 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 149
            original value before phred recall for SR was 800
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggga cacaatcgaa ttcaatctga cttatgaact catgaagaac gccgtggaca
       61 ttctggacgt gatgggactt cacttcgcaa tttcacactt tctcgcaaga aagggagaag
      121 tccggatgtt gactgcggga cttggctggg ccggtgccca ctcgcttctc acctactttg
      181 ttgtgcttgt agttggaacc cgaacaactg ggttcacttg gcgctatatc cagacagcaa
      241 ttgaatcaaa ttacgatttg gtgttttacc tttcaatggc gtcactagtt tggatcttca
      301 atcgaaatga tctcatcgtt cagtcaaaat atctcgcatg cttcctgttg atgacttgcg
      361 tcttacactc tgccatttgc caatctctct acttccattt cgctctacgt tcttggaaat
      421 taattgctgc tcagggagtt tttacctttg gactagcatt tttgacggtg gtagcatact
      481 cccggagttg ggctgatatc aatggattct atggcccaat aattcgattt taatttttt
//