LOCUS       EY190686                 456 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01a01.g2 R.similis_EST_RSAB Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190686
VERSION     EY190686.1  GI:159493361
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 849 was extended to value 850 (,)
            putative full insert read: vector-to-vector length is 576 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 729
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggcat cttcgacctg gcagagaact gtcgcctgta gcccttttca agtaggctgt
       61 tcaacaattc cccgctgtct tccctcagct gggaccatcc tctccgcctt cggctgcagc
      121 ccaacgagta gacgagtgag actgactgag cgatgaaggg aacagcgctc aacagtgttc
      181 tggggtcgga gtgcccaaac gagagccatc gctgggctgt gaccaacagg aaggcgatcg
      241 cacaaactgc ggcaaatgca agccagacga tggtgccgag ggagtgggcg atgctcaaaa
      301 ctgctggaaa tgcgtagcga ggatggaatg gcatcgtcat ttggagcaga agtgcgattg
      361 ccgtgagcga cgagcaaggg cgatgctatt gcccgttacg gcggcgatga ccataaaaag
      421 gagcagagtg aggtcggaaa gagtcaaaaa gaggag
//