LOCUS       EY190677                 368 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01c11.g2 R.similis_EST_RSAB Radopholus similis cDNA 5'
            similar to ref|NP_506513.1| TRNA Methylase TRM-1 (trm-1)
            [Caenorhabditis elegans] sp|Q23270|TRM1_CAEEL
            N(2),N(2)-dimethylguanosine tRNA methyltransferase
            (tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
            2,2-dimethylguanosine-26 methyltransferase) (tRNA(m(2, mRNA
            sequence.
ACCESSION   EY190677
VERSION     EY190677.1  GI:159493352
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 848 was extended to value 848 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 151
            original value before phred recall for SR was 554
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaaa ggcgattaag actgacgcgc cactcacatt tctgtgggac atctgccgtg
       61 tgtgggcaaa gaagtcaggg gtggacgcgg acaaacttgg atcagagtcg gccggaaggc
      121 gcattttgag tcaggaaccc agattcgaaa taaacttcgg gctgcatccg gacgccgttc
      181 ctcaaagcaa agcggtccag cttgttcgct ttccggataa taagggaaaa aactggggac
      241 cgaaggccaa ggccaaagga tcggtgagct caactacggc cgcctttgtg aagtgattct
      301 tccccaattc tcaaacttta ttttgttgag gccattgaaa aataatggaa taacaacaaa
      361 aaaaaaaa
//