LOCUS       EY190670                 304 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01g03.g2 R.similis_EST_RSAB Radopholus similis cDNA 5'
            similar to ref|ZP_00091246.1| hypothetical protein [Azotobacter
            vinelandii], mRNA sequence.
ACCESSION   EY190670
VERSION     EY190670.1  GI:159493345
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 499
            PolyA tail found inside read...SR being truncated to 455
            Potential full insert read...vec-to-vec length: 422
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggaag gaataaaggt agcataacaa aaaatcatga atgatttgaa aaaagcatga
       61 aatagaagaa atgaatccta taggccaacc ttttgacagt aacctacatc aagctgtaat
      121 agaaaaagag gatgatgaaa aagagcctgg aactataata gaagttttac aaactggtta
      181 cgtaatcaaa aaaaggctgc ttcgtccggc aatggtaatt atttctcaaa aatcagaaag
      241 ttcttcgcaa gaagattcta aggaaaatta atgatcgctt ttgcaggtat tcagccaagt
      301 gtga
//