LOCUS       EY190653                 443 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01d05.g2 R.similis_EST_RSAB Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190653
VERSION     EY190653.1  GI:159493328
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 879 was extended to value 879 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 153
            original value before phred recall for SR was 641
            PolyA tail found inside read...SR being truncated to 595
            Potential full insert read...vec-to-vec length: 580
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccggggaa tgtgggtcag ccgaacctaa ggcaccggct aacgccgtca cacactacgg
       61 ggaagactgg tgctcgcact tgtccacccg gttgccgaaa gggaagtggg ttaacattcc
      121 cacgccaggc tggggagacg gccgttcgca agagcgacta cgcgtggcga cacaaatgaa
      181 ctcgcagacg ttgtgcggag tccgggccag agttctcttt tctggtttag ggcgccagtc
      241 cctggagttg gctcagcctg agatagggac ggcgcgcccg taaagcaccg cggttcctgt
      301 ggtgtcccaa gcgctgcgca tgaccctcga aaatgcgagg gaagcgttgt gaatctcccg
      361 cctgctcgta ccgatatccg cagcaggtct ccaaggtgaa cagcctctag tcgatagaat
      421 aatgtaggta agggaagtcg gca
//