LOCUS       EY190643                 256 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa01f05.g2 R.similis_EST_RSAB Radopholus similis cDNA 5',
            mRNA sequence.
ACCESSION   EY190643
VERSION     EY190643.1  GI:159493173
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 923 was extended to value 924 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 150
            original value before phred recall for SR was 451
            PolyA tail found inside read...SR being truncated to 407
            Potential full insert read...vec-to-vec length: 392
            Seq primer: -21UPpOT.
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 ggccgggggg ggcatttgta ttgcggcgtg agaggtgaaa ttcttggacc gccgcaagac
       61 aaactacagc gaaagcactt gccaagaatg tcttcattaa tcaagaacga aagtcagagg
      121 atcgaaggcg atcagatacc gccctagttc tgaccgtaaa cgatgccaac tcgcgatccg
      181 ccggcggagc atttcgccct ggtggggagc gtcccggaaa cgaaagtctt ccggttccgg
      241 gggaagtatg gttgta
//