LOCUS EY190603 453 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa04e08.b1 R.similis_EST_RSAB Radopholus similis cDNA 3' similar to ref|NP_648923.1| CG9710-PA [Drosophila melanogaster] gb|AAL49107.1| RE55422p [Drosophila melanogaster] gb|AAF49407.2| CG9710-PA [Drosophila melanogaster], mRNA sequence. ACCESSION EY190603 VERSION EY190603.1 GI:159493133 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 453) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 55 original value before phred recall for SR was 921 COMM possible reversed clone; protein similarity on negative strand Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..453 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aggcggccga catgtcccat aagggggttt tttttttttt ttttgacaaa tggataccct 61 tttaggggtt aaaataaatc cggtgcttta actgattcga aaaataacaa aatttagaga 121 gccaccaata tttccgaagt gaccaattgg atactccgcc atcgtttacc tgaactttgc 181 ctgccaaaag accatttcag ggtgttgcgc cctaaacttg ttgagaatgt cttgtttctt 241 tttctcttcc gactaaggaa gtccaacttc cttatggcgc tggtctctac atcatcttta 301 ccaccatcga gcgagtttcc ccatccaaat ccgaaagctt gaagttctcc ggctgcacct 361 tctgagtgtc gatctcaggg tccctccccc tcagcttgat ccaccaatgc atcaggttta 421 tcttctccag ggaaagaaat actgcccact tat //