LOCUS       EY190598                 483 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa04e02.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to emb|CAC28353.1| putative calponin [Meloidogyne
            incognita], mRNA sequence.
ACCESSION   EY190598
VERSION     EY190598.1  GI:159493128
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 57
            original value before phred recall for SR was 537
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga atgtttcact caggggttta tttttttttt tttctttttt gggccccatt
       61 ggagggggct cgccatttgc aaaatgttgt caagtcttca gcatcgatcg gtggggagag
      121 gaggagaatt tttagcagcg gaggagaagt aggggggatg gacatatttg gtggttgtca
      181 ggcaatggcc aaatcagtgc acgaggagag aggggacagg ggacggacag ttcgccattc
      241 cgtggacagt tttcattccg cttctgtttc ttgtgcccgc gggacagtcg cctcctcttc
      301 agaggcggtc cagcgagatt tctgcctcct cggggtactc cagctcccaa atgcgcttca
      361 agtgcttgcc cttcacgtca cgcggcgtac cgaagccggt catcagcttt tgcgagtctc
      421 ccttgttcca tccggcttgg gacgggatga tcgcctccga cttcttcatg tcgcccggcc
      481 gta
//