LOCUS       EY190592                 611 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa04e06.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to ref|NP_502317.1| Proline HYdroxylase PHY-2 (61.5 kD)
            (phy-2) [Caenorhabditis elegans] sp|Q20065|P4H2_CAEEL Prolyl
            4-hydroxylase alpha-2 subunit precursor (4-PH alpha-2)
            (Procollagen-proline,2-oxoglutarate-4-dioxygenase alpha-2 subunit)
            pir|T21816, mRNA sequence.
ACCESSION   EY190592
VERSION     EY190592.1  GI:159493122
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 890 was extended to value 891 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 55
            original value before phred recall for SR was 664
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcttt ttagggtttt tttttttttt ttttaactca aagttatcct
       61 tttaggaggc gaacagaaaa cggtgctgca gtgaaattgt tgcgaacgtg agcactggat
      121 cagcataact tttcatcttc acgtgtaatc aaaaccaaca ctgatgaata ttttgtggct
      181 aataagtaat agtaatagtg cacccgaaca aatgttagga aatgtcttta tcgatcacaa
      241 cggacgcgta tcacccacaa acctttcatt tacgccttgt tttagggcgc aaggacggat
      301 gaattcttga ccgcgctcat gaatccactt gttggacacc catttgacac cagtcaacac
      361 cggacacgcc gcatgtcgtg tgcgtagatc gccctcccca tctctttgca gattgtacca
      421 aaacagcgca tcatggagtg aaggtgcaac accagtctcc aattcggtgt atactgtaag
      481 tccacctttc ttaggctgcg acatgtaaaa taagacggta gccacccgat tcccttttcc
      541 cttatacgga tttttttcgc cacgccttgc gaagtcaaag tgtgggtcgt aatgtccgcc
      601 gatcccggcc g
//