LOCUS       EY190585                 385 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa03c09.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to ref|NP_498776.1| Heat shock protein HSP-12.2 (hsp-12.2)
            [Caenorhabditis elegans] sp|P34328|YKZ1_CAEEL Hypothetical protein
            C14B9.1 in chromosome III pir|S44755 C14B9.1 protein -
            Caenorhabditis elegans gb|AAA27953.1| Heat shock protein protein
            12, mRNA sequence.
ACCESSION   EY190585
VERSION     EY190585.1  GI:159493115
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            putative full insert read: vector-to-vector length is 497 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 58
            original value before phred recall for SR was 555
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aaagacaata atagaagcgg agttggcact gttaactttg aagagcaata tttatctttt
       61 ctccgtccaa cacgatcgat tcatttcact gattggtggt cttccacgct cacttcttct
      121 gaccggtgac gcgcaacact ccgttctgcg tcagatgcga cttgatggcg gccgtgttca
      181 cgctgtcggg cagcttgtag cagcggttga tctcgcgact gatcgagccg tgctggtcgc
      241 tgcgctgttc gtgcttgcag tgaatggtca agtggtcgcc gactaccttg acctcaattt
      301 cgtttggcgt gaagtaggcc acgtccggtc ccacctccca cttgtccggc gtgtcgtgca
      361 ccttcaccac tccatcgttg tgctg
//