LOCUS EY190560 394 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa03c12.b1 R.similis_EST_RSAB Radopholus similis cDNA 3', mRNA sequence. ACCESSION EY190560 VERSION EY190560.1 GI:159493090 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 394) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 56 original value before phred recall for SR was 844 Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..394 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aatcatggcg gatatacagt atcagtatgc ctacttatac tcactctgac ttacacccac 61 ttccactata cacactacac cgtgggtaca agtgttcgag taaacattat ttggtttgat 121 tatgaactac agtttcttca ttggtgttct ttttctcatc agatcggttt tccctgcatg 181 aatgcttcga tggcgtgcaa cagttcaaaa cctttcttct gtggcaccat gtgtcctgca 241 ccgcggattg tgatgaaagt taggcctccc ttgtacacag ttttgaatcc gcccacttgt 301 ccacggtaaa cccatggctt ggccggcgtc tccacctcat ggccgaggct ggcagcaaac 361 tttgatccga ggatcaaatt gcatcgcatg tccg //