LOCUS       EY190557                 597 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa03c07.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to gb|AAL40720.1|AF402771_1 calreticulin [Meloidogyne
            incognita], mRNA sequence.
ACCESSION   EY190557
VERSION     EY190557.1  GI:159492848
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 55
            original value before phred recall for SR was 907
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgtttctt tttgggggtt tttttttttt tttttctaat tagggatcct
       61 tttttgggga cgcagagtaa gaaattcagc atgctataac aattttagtc tcaaagggat
      121 caatcaaaac ctttgatgtt gagagaaaag acaatttaat tgcaaaataa attatttggt
      181 tggatgcaga taaatacgag ggggatggcg aggagaccat tgaataattt ctaacagaaa
      241 taggcttgat gtctgtgtcc attccagatt ggttgtaagt agaaattgaa acaaacaaat
      301 attctttgat ggcccagaaa ggctgatctg gtggtaaagc gggtcaaggc aagtccaaaa
      361 atctgcttgt cggtcttaga gctcatcatg gtcctctttc tcctcctctt tctcatcttc
      421 gtcctcatct tccttctcgt cctttttctt ggactcttcc tcctccttcc gcttcttctc
      481 ttcctcttcg aattttttcc gctcctcttc gtcatgtgcc tccttctgct tcttctcagc
      541 atcacgcatc ggctcaaagg tttccttggc gtgcgtcttt gcatcgtcga tgttgtc
//