LOCUS       EY190556                 295 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa03f09.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to gb|AAL78225.1|AF345797_1 putative pyruvate dehydrogenase
            [Heterodera glycines], mRNA sequence.
ACCESSION   EY190556
VERSION     EY190556.1  GI:159492847
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 58
            original value before phred recall for SR was 583
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 tggtcttggg ggaaacataa attgacacac aatgcgggat aggagagcaa accaaatttt
       61 ttctactttc aaatgtgatt tctaatcggt tttaaattaa tctagggtga ctctgctagt
      121 atgcctttgg ctctctgccc atctttctga acacgtcgga ggtcgtaacg taaggctgca
      181 caaccgtgtt gtcgatggtc gtgcccctga ccaactgggc gggagtgttc cagtagagat
      241 cccagtacaa aacgtccagg ggcaaaacgt catcggtcag cgccaccttc agggc
//