LOCUS       EY190553                 594 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa03d08.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to ref|NP_505977.1| Biotin-requiring PYruvate Carboxylase
            1; cadmium-responsive gene (129.3 kD) (pyc-1) [Caenorhabditis
            elegans] pir|T20346 pyruvate carboxylase (EC 6.4.1.1) D2023.2
            [similarity] - Caenorhabditis elegans emb|CAB02872.1| C. elegans
            PYC, mRNA sequence.
ACCESSION   EY190553
VERSION     EY190553.1  GI:159492844
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 57
            original value before phred recall for SR was 922
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcctt tataggtttt tttttttttt ttttccataa aagggatcca
       61 tatggaggag tacttatctg acatccaaat aagtgttttt gagaatagaa ttttggaggg
      121 ttcgcaatta attttatgga attggagaac aatggcatat aggcataaaa tgagcaagtg
      181 atcttaaacg taagaggggc tggggcaact agaaacatgt atctgctttc cctacatgtt
      241 ttgaattaga aatcttattg ctaaaagtcc cgaggaacct acgtacgtgt aaaatgtaaa
      301 aatgcattta aaaatagaag gattgggtag tgggcgagtt tttatggttc tactacgatc
      361 acaagatcac cgggcgcaac cttctcacct ttttgcaagc aaatactctt aactgtgccg
      421 gagatcgggg agtcaatgtt catttccatt ttcattgcgg agagcacgaa gagggtctgc
      481 ttgggttgta ccttgtctcc tactttgacc ttgacttcca aaatctctcc gggcattggt
      541 gccccaatag atcccttcac cagcgtgttg gcctttggcc taacgactat gtct
//