LOCUS       EY190549                 538 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa03f08.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to sp|P41340|ACT3_LIMPO ACTIN 3 pir|S49480 actin 3 -
            Atlantic horseshoe crab emb|CAA86290.1| actin [Limulus polyphemus],
            mRNA sequence.
ACCESSION   EY190549
VERSION     EY190549.1  GI:159492840
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            original QR value of 889 was extended to value 889 (,)
            This trace has been recalled with phred
            original value before phred recall for SL was 57
            original value before phred recall for SR was 899
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttcctt ttggggtttt tttttttttt tcggtccata gagatccttt
       61 tgaggggcga ttgatccatg accacttctt atttaaacaa agaaagaaag atatgggttc
      121 gtgtccgttt ttgccatttg ccgttgtctt tcccttctta gaagcatttg cggtgaacaa
      181 tcgagggacc ccactcgtcg tactcctgct ttgagatcca catctgctgg aaggtagaga
      241 gcgaggccag gatggagcca cctatccaga cggagtactt gcgctcaggc ggcgcgatga
      301 tcttgatctt catggcggaa ggcgccagcg cggggatctc cttctgcaag cggtcagcaa
      361 ttcccgggta catgggggtg ccgccggaaa gcacggtgtt cgcgtacagg cccttacgga
      421 tgtcaatagt cccacttcat gatcgagttg taccaggtct cgaggatgcc ggccgactcc
      481 atgcccagga aagagggctg gaagagggcc tcgggacaac ggaagcgctc gtatccga
//