LOCUS EY190545 469 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa03c10.b1 R.similis_EST_RSAB Radopholus similis cDNA 3', mRNA sequence. ACCESSION EY190545 VERSION EY190545.1 GI:159492836 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 469) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 53 original value before phred recall for SR was 513 Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..469 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aggcggccga atgttcccct tatgggggtc tttttttttt ttttcaaaag ttgggacccc 61 tttaaggggt caaggatcat catcgtttgg caaaaagcat taattgtgtg tgctggggct 121 gtctgcgcct ttttcggctg ttcggccgcc actgccaatg tgctggaaca ctcgtgcgat 181 gaagtgctgc tgctgctgct gcggcgcttg ggctactgcc aacggctgct gcagccgctc 241 cgcacgccca aacccgtcct tcacttctgc gcactcttgc aacatctgct cctccgattg 301 ggcgccgacg gcggacaggc tccaattagg cgaactgcaa acccagcatt gtgtcactgt 361 ccggccttaa gcgagtaagc aaaaatacaa aaataggatc cgtggaatgc gggttggctc 421 ctctcgggag agttggcagc gcacaaacaa atgtgtgtcc ccggccgta //