LOCUS EY190543 596 bp mRNA linear EST 08-NOV-2007 DEFINITION RSAB-aaa03f03.b1 R.similis_EST_RSAB Radopholus similis cDNA 3', mRNA sequence. ACCESSION EY190543 VERSION EY190543.1 GI:159492834 KEYWORDS EST. SOURCE Radopholus similis ORGANISM Radopholus similis Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina; Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus. REFERENCE 1 (bases 1 to 596) AUTHORS Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J., Scott,A.L., McCarter,J.P. and Wilson,R. TITLE WashU Nematode EST Project JOURNAL Unpublished COMMENT Contact: Dr. Makedonka Mitreva WashU Nematode EST Project Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA Tel: 314 286 1800 Fax: 314 286 1810 Email: submissions@watson.wustl.edu Library material provided by Dr. Godelieve Gheysen Library constructed by Dept. of Molecular Biotechnology,Ghent University Library sequenced by Washington University Genome Sequencing Center This trace has been recalled with phred original value before phred recall for SL was 56 original value before phred recall for SR was 892 Seq primer: pDNR-LIB-reverse. FEATURES Location/Qualifiers source 1..596 /organism="Radopholus similis" /mol_type="mRNA" /db_xref="taxon:46012" /clone_lib="LIBEST_022112 R.similis_EST_RSAB" /dev_stage="mixed" /lab_host="DH5alpha" /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI" ORIGIN 1 aggcggccga catgttcttt ttaggggttt tttttttttt ttttggattc agggattttt 61 ttggggaaca catgcaccaa gaaccaataa aattaaaaaa aatcaaacga aatatagaaa 121 cgataaaaat ttgtgtcaat gagcgttctc gggggtcagt tgggttaatt cctcaattgg 181 tcaatagaag cactgcttca cgattccggc cgcaattgtt tcgccctggg cacgcattgc 241 cagtctgccc agcgacttgg tgcgtgcaaa cggctcaacc gggatgcccg actctgtgtg 301 cacctccacc agtgcgctga catttcgagg gagcaatctc tgaatcagca actccagctt 361 gatcgcattc gcctcggtca gctgtgcctc tagactgctg gaaaaccatc cgaccacttt 421 ctttttggcg ctgcgcaaca gtgtcggggg ccagatcgta agcagtgacg ggggcctccc 481 catttcgaaa accaaacgct taattcccgc ctcagccaaa atcttagtgg actccggatc 541 aaattttacc cccttcagct ccacacaaaa aagccatcca gtccatgcct ctctgt //