LOCUS       EY190540                 582 bp    mRNA    linear   EST 08-NOV-2007
DEFINITION  RSAB-aaa03d07.b1 R.similis_EST_RSAB Radopholus similis cDNA 3'
            similar to pir|F88577 protein R01H10.7 [imported] - Caenorhabditis
            elegans emb|CAA83465.1| Hypothetical protein R01H10.7
            [Caenorhabditis elegans], mRNA sequence.
ACCESSION   EY190540
VERSION     EY190540.1  GI:159492831
KEYWORDS    EST.
SOURCE      Radopholus similis
  ORGANISM  Radopholus similis
            Eukaryota; Metazoa; Nematoda; Chromadorea; Tylenchida; Tylenchina;
            Tylenchoidea; Pratylenchidae; Radopholinae; Radopholus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Mitreva,M., Fulton,L., Becker,M., Ronko,I., Theising,B., Martin,J.,
            Scott,A.L., McCarter,J.P. and Wilson,R.
  TITLE     WashU Nematode EST Project
  JOURNAL   Unpublished
COMMENT     Contact: Dr. Makedonka Mitreva
            WashU Nematode EST Project
            Washington University School of Medicine
            4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108, USA
            Tel: 314 286 1800
            Fax: 314 286 1810
            Email: submissions@watson.wustl.edu
            Library material  provided by Dr. Godelieve Gheysen Library
            constructed by Dept. of Molecular Biotechnology,Ghent University
            Library sequenced by Washington University Genome Sequencing Center
            This trace has been recalled with phred
            original value before phred recall for SL was 58
            original value before phred recall for SR was 636
            COMM   possible reversed clone; protein similarity on negative
            strand
            Seq primer: pDNR-LIB-reverse.
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Radopholus similis"
                     /mol_type="mRNA"
                     /db_xref="taxon:46012"
                     /clone_lib="LIBEST_022112 R.similis_EST_RSAB"
                     /dev_stage="mixed"
                     /lab_host="DH5alpha"
                     /note="Vector: pDNR-LIB; Site_1: SfiI; Site_2: SfiI"
ORIGIN      
        1 aggcggccga catgttccct agaggggttc tttttttttt ttttgcaggt atgcatccct
       61 atgggagggt taagaggcga tcaaatttgt aaatgtgcca aacgggggcc gtagtgcctt
      121 gggaaggaat ttcatttgga aagggctgaa cgcgaagaga ggccttccaa cgttttttct
      181 gcaattctcc cttctaactc cattcctccg cagacattcc acgatctcag acatctgttt
      241 attcgatatg ccacatgtct ctctcagaat cctcccctct tcaagtgtgg ccgccatcga
      301 agtccggtct tttccgcttt tgcaagagat tactggtact ccgccgagag cactgttaag
      361 agccatcact tgcgagaaaa tcagaatgtt ctttcgagag ggacaggcag acaccgagca
      421 actgctctac aaggcctgca ttctccattt gtgcatcgac agccgaaacc aagagagaat
      481 attcgaccat ttggcgaagg gcttctctgt tcacttcctc ttcaaaactg atatcgccaa
      541 tcgacgcagc cagagaggct tcgtgattca ctcccccggc cg
//